pcdna3 1 egfp Search Results


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Notch activity as a potential readout of nuclear position. (A) nort:d2GFP expression reports Notch activity in the neural tube. Arrowhead indicates GFP-negative basally localized neurons. Scale bar: 10 µm. (B) Notch activity (green) is significantly inhibited in progenitors that are adjacent (and basal) to an arrested mitotic cell (magenta) (P<0.01, n=15 for control, n=12 for dnPLK1). (C) Inhibition of Notch signaling (via microinjection of <t>hsp:dnMAML1-P2A-h2bCherry2)</t> promotes differentiation compared with a control injection (hsp:h2bCherry2) (P<0.001 for n=11). Scale bar: 10 µm.
P2a Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Notch activity as a potential readout of nuclear position. (A) nort:d2GFP expression reports Notch activity in the neural tube. Arrowhead indicates GFP-negative basally localized neurons. Scale bar: 10 µm. (B) Notch activity (green) is significantly inhibited in progenitors that are adjacent (and basal) to an arrested mitotic cell (magenta) (P<0.01, n=15 for control, n=12 for dnPLK1). (C) Inhibition of Notch signaling (via microinjection of <t>hsp:dnMAML1-P2A-h2bCherry2)</t> promotes differentiation compared with a control injection (hsp:h2bCherry2) (P<0.001 for n=11). Scale bar: 10 µm.
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Notch activity as a potential readout of nuclear position. (A) nort:d2GFP expression reports Notch activity in the neural tube. Arrowhead indicates GFP-negative basally localized neurons. Scale bar: 10 µm. (B) Notch activity (green) is significantly inhibited in progenitors that are adjacent (and basal) to an arrested mitotic cell (magenta) (P<0.01, n=15 for control, n=12 for dnPLK1). (C) Inhibition of Notch signaling (via microinjection of <t>hsp:dnMAML1-P2A-h2bCherry2)</t> promotes differentiation compared with a control injection (hsp:h2bCherry2) (P<0.001 for n=11). Scale bar: 10 µm.
Pcdna3 1 Dcas9 2xnls Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Notch activity as a potential readout of nuclear position. (A) nort:d2GFP expression reports Notch activity in the neural tube. Arrowhead indicates GFP-negative basally localized neurons. Scale bar: 10 µm. (B) Notch activity (green) is significantly inhibited in progenitors that are adjacent (and basal) to an arrested mitotic cell (magenta) (P<0.01, n=15 for control, n=12 for dnPLK1). (C) Inhibition of Notch signaling (via microinjection of <t>hsp:dnMAML1-P2A-h2bCherry2)</t> promotes differentiation compared with a control injection (hsp:h2bCherry2) (P<0.001 for n=11). Scale bar: 10 µm.
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Notch activity as a potential readout of nuclear position. (A) nort:d2GFP expression reports Notch activity in the neural tube. Arrowhead indicates GFP-negative basally localized neurons. Scale bar: 10 µm. (B) Notch activity (green) is significantly inhibited in progenitors that are adjacent (and basal) to an arrested mitotic cell (magenta) (P<0.01, n=15 for control, n=12 for dnPLK1). (C) Inhibition of Notch signaling (via microinjection of <t>hsp:dnMAML1-P2A-h2bCherry2)</t> promotes differentiation compared with a control injection (hsp:h2bCherry2) (P<0.001 for n=11). Scale bar: 10 µm.
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Notch activity as a potential readout of nuclear position. (A) nort:d2GFP expression reports Notch activity in the neural tube. Arrowhead indicates GFP-negative basally localized neurons. Scale bar: 10 µm. (B) Notch activity (green) is significantly inhibited in progenitors that are adjacent (and basal) to an arrested mitotic cell (magenta) (P<0.01, n=15 for control, n=12 for dnPLK1). (C) Inhibition of Notch signaling (via microinjection of <t>hsp:dnMAML1-P2A-h2bCherry2)</t> promotes differentiation compared with a control injection (hsp:h2bCherry2) (P<0.001 for n=11). Scale bar: 10 µm.
Pcdna3 1 Dcas9 Dmq1 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Notch activity as a potential readout of nuclear position. (A) nort:d2GFP expression reports Notch activity in the neural tube. Arrowhead indicates GFP-negative basally localized neurons. Scale bar: 10 µm. (B) Notch activity (green) is significantly inhibited in progenitors that are adjacent (and basal) to an arrested mitotic cell (magenta) (P<0.01, n=15 for control, n=12 for dnPLK1). (C) Inhibition of Notch signaling (via microinjection of <t>hsp:dnMAML1-P2A-h2bCherry2)</t> promotes differentiation compared with a control injection (hsp:h2bCherry2) (P<0.001 for n=11). Scale bar: 10 µm.
Pcdna3 1 Dcas9 Mq1 Q147l Egfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Notch activity as a potential readout of nuclear position. (A) nort:d2GFP expression reports Notch activity in the neural tube. Arrowhead indicates GFP-negative basally localized neurons. Scale bar: 10 µm. (B) Notch activity (green) is significantly inhibited in progenitors that are adjacent (and basal) to an arrested mitotic cell (magenta) (P<0.01, n=15 for control, n=12 for dnPLK1). (C) Inhibition of Notch signaling (via microinjection of <t>hsp:dnMAML1-P2A-h2bCherry2)</t> promotes differentiation compared with a control injection (hsp:h2bCherry2) (P<0.001 for n=11). Scale bar: 10 µm.
Selection, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc paper n a pcdna3 1 flag nls ms2 gfp addgene
Notch activity as a potential readout of nuclear position. (A) nort:d2GFP expression reports Notch activity in the neural tube. Arrowhead indicates GFP-negative basally localized neurons. Scale bar: 10 µm. (B) Notch activity (green) is significantly inhibited in progenitors that are adjacent (and basal) to an arrested mitotic cell (magenta) (P<0.01, n=15 for control, n=12 for dnPLK1). (C) Inhibition of Notch signaling (via microinjection of <t>hsp:dnMAML1-P2A-h2bCherry2)</t> promotes differentiation compared with a control injection (hsp:h2bCherry2) (P<0.001 for n=11). Scale bar: 10 µm.
Paper N A Pcdna3 1 Flag Nls Ms2 Gfp Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Notch activity as a potential readout of nuclear position. (A) nort:d2GFP expression reports Notch activity in the neural tube. Arrowhead indicates GFP-negative basally localized neurons. Scale bar: 10 µm. (B) Notch activity (green) is significantly inhibited in progenitors that are adjacent (and basal) to an arrested mitotic cell (magenta) (P<0.01, n=15 for control, n=12 for dnPLK1). (C) Inhibition of Notch signaling (via microinjection of <t>hsp:dnMAML1-P2A-h2bCherry2)</t> promotes differentiation compared with a control injection (hsp:h2bCherry2) (P<0.001 for n=11). Scale bar: 10 µm.
Pcdna 3 1 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Notch activity as a potential readout of nuclear position. (A) nort:d2GFP expression reports Notch activity in the neural tube. Arrowhead indicates GFP-negative basally localized neurons. Scale bar: 10 µm. (B) Notch activity (green) is significantly inhibited in progenitors that are adjacent (and basal) to an arrested mitotic cell (magenta) (P<0.01, n=15 for control, n=12 for dnPLK1). (C) Inhibition of Notch signaling (via microinjection of hsp:dnMAML1-P2A-h2bCherry2) promotes differentiation compared with a control injection (hsp:h2bCherry2) (P<0.001 for n=11). Scale bar: 10 µm.

Journal: Development (Cambridge, England)

Article Title: Feedback between tissue packing and neurogenesis in the zebrafish neural tube

doi: 10.1242/dev.157040

Figure Lengend Snippet: Notch activity as a potential readout of nuclear position. (A) nort:d2GFP expression reports Notch activity in the neural tube. Arrowhead indicates GFP-negative basally localized neurons. Scale bar: 10 µm. (B) Notch activity (green) is significantly inhibited in progenitors that are adjacent (and basal) to an arrested mitotic cell (magenta) (P<0.01, n=15 for control, n=12 for dnPLK1). (C) Inhibition of Notch signaling (via microinjection of hsp:dnMAML1-P2A-h2bCherry2) promotes differentiation compared with a control injection (hsp:h2bCherry2) (P<0.001 for n=11). Scale bar: 10 µm.

Article Snippet: The hsp:mTagBFP2-dnPLK1-2A-p50 was similarly made, but with two extra pieces: the P2A sequence (Addgene, #52421) and the p50 sequence (amplified from zebrafish cDNA).

Techniques: Activity Assay, Expressing, Control, Inhibition, Microinjection, Injection